Diet and nutrition is a crucial part of reproductive health. Deficiency of minerals and micronutrients has been reported to impair the process of spermatogenesis (Smith and Akinbamijo, 2000). Historically, salt has been used by women on their husbands to increase their libido (Moiner and Drueke (2008). The present study was designed to determine the effect of low salt diet and high salt diet on sperm parameters, oxidative status and reproductive hormone levels of male rats. Eighteen healthy Sprague Dawley male rats weighing between 120- 130g were used. The rats were divided into three groups of six rats each. Group I: (control) received 0.3% salt diet, Group II: low salt (received 0.14% salt diet), and group III: high salt (received 8% salt diet). All animals were treated for six (6) weeks after which they were sacrificed by cervical dislocation. Sperm analysis was done on sample derived from the cauda epididymis by conventional methods. Sperm motility was done and expressed as percentage (Zemjanis 1977). The reduced glutathione (GSH) content of the testis and epididymis homogenate were determined using the method described by Van Dooran(1978). Malondialdehyde (MDA) was determined based on its interaction with thiobarbituric acid (TBA).The activity of the superoxide dismutase (SOD) enzyme in the testis homogenate was determined according to the method described by Sun and Zigman(1978). Serum samples were also collected and an enzyme linked immunoassay (EIA) system was employed to determine testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels. Values are means ± S.E.M., compared by ANOVA. This study reports a decreased sperm count in the low salt diet rats (44.06 ± 1.3) and increased sperm count in the high salt diet treated rats (59.6±2.3) Vs control (51.45 ± 2.2). Both low salt and high salt diet treated rats had a significant increase in percentage abnormal sperm cells when compared with control values at P< 0.05 (12.6 ± 1.2, 9.9 ± 1.3 respectively Vs 5.46 ± 0.8) The level of MDA significantly increased while GSH significantly decrease in the epididymis of both low and high salt treated diet (P< 0.05). Also a significant decrease in the activities of SOD and CAT in both the testes and the epididymis were observed in both treated groups. The increased MDA levels in the epididymis and decrease in antioxidant status in both salt diets indicates the presence of oxidative stress thus both low and high salt diet might play a negative role in male rats reproduction/fertility.
Physiology 2012 (Edinburgh) (2012) Proc Physiol Soc 27, PC335
Poster Communications: Reproductive parameters and oxidative status of male Sprague Dawley rats treated with low and high salt diet
B. O. Iranloye1, G. Oludare1, O. Morakinyo1, N. Esume1, L. Ekeh1
1. Physiology, Collegeof Medicine, University of Lagos, Lagos, Lagos, Nigeria.
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Where applicable, experiments conform with Society ethical requirements.