The progressive muscle wasting that occurs with age is called Sarcopenia. Ageing is synonymous with frailty, caused by the loss of strength and movement. This deterioration usually starts in adults aged approximately forty and up to 40% loss in muscle mass can occur. There are many purported reasons for Sarcopenia such as the reduction in exercise, changes in hormone levels, denervation of muscle, muscle remodeling, decrease in mitochondrial regenesis and decreased rates of protein synthesis. The mapping of the Aged muscle Phosphoproteome will play a substantial part in identifying novel disease markers that occur during ageing. To identify these Biomarkers, total differential proteomic analysis of Wistar rats was carried out. The separation of full cell protein lysate was carried out by two-dimensional gel electrophoresis. Then the gels were fluorescently stained and imaged to reveal phosphorylation levels using a fluorescent phosphosensor dye (Pro-Q Diamond dye) and subsequent MALDI-ToF mass spectrometry analyses to identify differentially expressed proteins.