The human cutaneous microcirculation has been considered as a useful “window” to assess microvascular function, especially because of the possibility of using non-invasive technologies. Whenever measuring skin perfusion, it is advisable to measure skin temperature as well, in order to control the interference of thermoregulatory phenomena due to changes in ambient temperature. When performing measurements in an environment of stable external temperature, changes in skin temperature should reflect changes in skin perfusion. In fact, depending on the sensitivity of the sensors, it is possible that changes in skin temperature may reflect physiological mechanisms regulating perfusion. However, the spectral organization of continuous skin temperature signals remains poorly understood. Therefore, this study aimed to compare the frequency spectra of skin perfusion and skin temperature signals obtained from healthy subjects. Twenty healthy subjects (21.0 ± 3.0 y.o., both sexes) participated in this study after giving informed consent. After acclimatizing to room conditions, subjects performed one of two protocols while sitting upright – 10 subjects were subjected to a suprasystolic limb occlusion protocol (SLO, 5 min baseline, 5 min occlusion of a random arm, 5 min recovery); the other 10 subjects performed a postural modification (5 min with both hands at heart level, 5 min with a random hand placed 40 cm below heart level, 5 min recovery in the initial position). In both protocols, two variables were quantified in the index finger of the tested (occluded or lowered) limb – skin perfusion was quantified in the distal phalanx with a photoplethysmography (PPG) sensor, and skin temperature was quantified in the middle phalanx with a negative temperature coefficient (NTC) thermistor. Nonparametric statistics were used for comparisons between phases and signals (p<0.05). In the SLO protocol both perfusion and temperature decreased significantly due to the mechanical compression of the brachial artery. In the hand lowering protocol, however, perfusion decreased significantly due to the venoarteriolar reflex, but not significant changes were observed for skin temperature. These results show that the NTC thermistor was less sensitive to the physiological challenges than the PPG sensor. Both signals were decomposed with the wavelet transform to obtain their respective frequency spectra. The spectral organization of the PPG signal has already been proposed to contain several components – cardiac, respiratory, myogenic, sympathetic, endothelial NO-dependent and endothelial NO-independent. The spectra of the temperature signal revealed components in the same frequency intervals as the PPG signal. In fact, the dominant frequency of each observed component was generally coincident between signals, although appreciable differences in terms of skewness and kurtosis were identified for the regions of cardiac and respiratory components. These results suggest that skin temperature signals might have the same physiological origins than PPG signals and, consequently, might be useful to explore the dynamics of perfusion regulation.