Sweat rate is higher in perimenopausal women than in premenopausal and postmenopausal women (Minkin et. al., 1997) and excessive sweating during menopause depletes some of the body’s essential nutrients, especially potassium (Balch and Balch, 1999). In this study, we assessed whether there is an association between sweat rate (SR), sweat volume (SV) and sweat potassium concentration (S[K+]) in premenopausal (preM), perimenopausal (periM) and postmenopausal (postM) women after a moderate exercise. Thirty (30) healthy women comprising of preM (aged 22.5±0.8yrs, n=10), periM (aged 46.5±1.1yrs, n=10) and postM (aged 52.2±0.9yrs, n=10) participated in the study. Participants were adequately informed of the experimental procedures and they all consented to it. TP was rated using the Visual Analogue Scale, VAS. Sweat was obtained with the sweat suction apparatus from a 120cm2 circular area marked on the skin of the face and neck (Ugwu and Oyebola, 1996), after a moderate exercise (a 15min walk on the treadmill) at a speed of 4.2 km/h at 270C, with the start time noted and SV and [K+] measured. SR was determined using the formula: (volume of sweat collected)/(exercise time) . The results show that periM women exhibited a significantly higher SR (0.12±0.01 ml/min vs preM – 0.07±0.02 ml/min vs postM – 0.06±0.01 ml/min) (p<0.05), but a significantly lower S[K+] (19.98±1.5 mmol/l vs preM – 23.37±1.3 mmol/l vs postM – 24.88±1.8 mmol/l) (p<0.05). However, there was no significant difference in the SV (periM – 1.7±0.2 ml vs preM – 1.3±0.4 ml vs postM – 0.9±0.1 ml). The linear regression plot showed a significant (p<0.05) inverse relationship (negative correlation) between S[K+] and SR, but the negative relationship between S[K+] and SV was not significant, i.e. increase in SR and/or SV produced a decrease in S[K+]. In conclusion, these data indicate that although excessive sweating can lead to depletion of the body’s potassium concentration, the sweat potassium concentration decreases with increasing sweat rate and volume especially in perimenopausal women during moderate exercise.