Background: Antimicrobial peptides (AMPs) are important effectors of the innate defense system. Cathelicidins, (CRAMP in mouse/rat, LL-37 in human) is one of the two major classes of AMPs in humans.The upregulation of LL-37 synthesis is a novel non-antibiotic approach to prevent or treat infectious diseases. Butyrate was found to induce Cathelicidin expression. Gum Arabic (GA), an exudate from Acacia senegaltree, is known for its prebiotic effects. Fermentation of GA by colonic bacteria increases serum butyrate concentrations. This study was conducted to investigate if GA supplementation can increase Cathelicidin expression in macrophages. Methods:The study was an in-vivo experiment in mice. Thirty mice were randomly divided into three groups, of ten mice per group. The two intervention groups received GA dissolved in drinking water in two different concentrations (15% and 30%)for 28 days. The third group served as a control. Blood was collected on Day 29 to isolate peripheral blood mononuclear cells (PBMC) which were cultured to obtain monocyte derived macrophages (MDMs).The transcription level of CRAMP was determined in MDMsby qPCR. Results: We detected a significant increase in CRAMP expressionin MDMsfollowing 28 days of 15% but not 30% GA supplementation. Conclusion: GAsupplementation can induce Cathelicidin expression in MDMs and the effect is dose dependent
Physiology 2021 (2021) Proc Physiol Soc 48, PC037
Poster Communications: The Effect of Gum Arabic Supplementation on Cathelicidin expression in Monocyte Derived Macrophages in Mice
Nagat bashir1, Rehab Badi2, Tahani Mohammeddeen3, Khalid Enan4, Amal Saeed5
1 Department of Physiology, Faculty of Medicine, Red Sea University, Portsudan, Sudan. Email Address: Nagatb05@gmail.com. , Portsudan, The Sudan 2 Physiology department, faculty of medicine, king khalid University , Saudi Arabia, Abha, Saudi Arabia 3 Department of Microbiology, Faculty of Medicine, Red Sea University,Portsudan, Sudan, Portsudan, The Sudan 4 Department of Virology, Central Laboratory, Ministry of Higher Education and Scientific Reaserch, Department Khartoum, Sudan, khartoum, The Sudan 5 Department of Physiology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan. , khartoum, The Sudan
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