It has been suggested that the elastic properties of kinesiology taping (KT) contributes to improvements in musculoskeletal pain and function. Delayed onset muscle soreness (DOMS) is an experimental model used to evaluate the effect of treatments on musculoskeletal pain in human participants. To date, studies using DOMS have found that KT reduced musculoskeletal pain and improved function. These studies have used isokinetic dynamometry which measures maximal voluntary contraction of muscle groups and relies on the participant’s ability to exert maximal voluntary effort. Tensiomyography (TMG) has been used to assess the contractile properties of muscle in response to electrical stimuli, such as maximal muscle displacement (Dm) and contraction time (Tc). An advantage of TMG is that the technique does not rely on volitional effort. There is a paucity of studies that have assessed the effect of KT on contractile properties of individual muscles in response to an involuntary contraction. The aim of this study was to compare the effects of KT, non-elastic surgical tape (NET) and no tape (nT) on musculoskeletal pain and muscle contractile properties of the biceps brachii, following the induction of experimental DOMS. This was a randomised, repeated measures, parallel group study. Participants received 1 of 3 possible interventions (KT, n=10; NET, n=10; nT, n=10). Day 1 consisted of baseline (pre-DOMS) measurements, induction of DOMS (eccentric muscle exercises), application of intervention (10mins) and immediate post-intervention measurements. Tape was then re-applied for participants in the tape groups and remained in-situ until the next study visits on Day 3 and Day 5. At these visits, measurements were taken 20mins after tape had been removed. Primary measurements at each study visit were: pain intensity during full flexion and extension (mPI, numerical pain rating scale, 0=no pain, 10=worst pain imaginable), and Dm by incrementally increasing the amplitude of the electrical stimulus until Dm reached a plateau. Primary outcomes were differences in mPI and Dm at Day 3 relative to baseline (Day 3 – baseline) and analysed using a one-way ANOVA. Thirty participants completed the study (mean ± SD age: 25.97 ± 4.98 years; height: 171.58 ± 8.96 cm; weight: 76.97 ± 15.47 kg; 16 females). Paired t-tests revealed that mPI increased (P = 0.00) and Dm decreased (P = 0.00) at Day 3 relative to baseline. There were no differences in the magnitude of this change between groups for mPI (mean ± SD KT = 5.00 ± 2.05, NET = 5.40 ± 2.50, nT= 5.20 ± 2.15, P = 0.92) or Dm (KT -2.33 ± 4.68, NET = -2.97 ± 4.51, nT = -3.23 ± 5.39, P = 0.91). In conclusion, KT and NET did not alter the increase in mPI and decrease in Dm associated with DOMS.