Resveratrol (3,5,4’-trans-trihydroxystilbene), a natural phytoalexin present in grapes, peanuts, mulberries and red wine, was shown to induce a number of endogenous antioxidants (1). Resveratrol (RVT) has additional pharmacological effects including anti-inflammatory properties, modulation of lipid metabolism and prevention of cancer (2, 3). The anti-inflammatory effect of RVT is related with its inhibitory action on oxidation, leukocyte priming and expression of inflammatory mediators. In our previous studies, we have also demonstrated that resveratrol improves renal damage in ischemia/reperfusion and sepsis-induced oxidative renal injury (4 , 5). The present study was aimed to investigate the possible protective effects of RVT against nicotine induced oxidative damage in urinary bladder, corpus cavernosum and kidney tissues. Wistar Albino rats were injected intraperitoneally for 21 days with either nicotine hydrogen bitartarate (0.6 mg/kg/day) or saline along with the oral administration of RVT (10 mg/kg/day) or saline. Then the rats were decapitated and trunk blood was collected. In the serum samples malondialdehyde (MDA), glutathione (GSH), BUN, creatinine, TNF-α levels and LDH activity were analyzed. Urinary bladder and kidney samples were taken for the measurement of tissue MDA and GSH levels, and myeloperoxidase (MPO) activity. Tissue samples were also examined histologically. Chronic nicotine administration caused a significant decrease in GSH levels and increases in MDA levels and MPO activity in both tissues, suggesting oxidative organ damage, which was also histologically verified. Furthermore, RVT restored the reduced GSH levels, while it significantly decreased MDA levels and MPO activity. Impaired renal function tests and elevated levels of LDH and TNF-α due to nicotine administration were reversed with RVT treatment. The present data suggest that RVT supplementation, possibly by its antioxidant effects, effectively counteracts the chronic nicotine toxicity and attenuates oxidative damage of bladder and kidney tissues, nominating resveratrol as a highly promising agent in avoiding the development of nicotine-induced toxicity in the urinary system.