Background: Electrogenic transmembrane integrity and its constituents are the basic features of the sperm membrane necessary for spermatogenesis, testicular metabolism and sperm fertilization potential; therefore, it is important to note that membrane surface responsible for ion exchanged and permeability modified upon oxidation and nitrosation. Objectives: The present study was designed to investigate the preventive effects of CoQ-10 and taurine supplement on transmembrane proton pump (ATPase) activitiesin CPZ-induced peroxinitrite formation in male wistar rats. Method: Thirty-Six male Wistar rats (150-200g) were divided into six groups (n=6) and treated daily for 56 days as follows: group 1 received distilled water (0.5 ml/kg) and served as normal control, group 2 received PG (0.5 ml/kg of 20%) and served as vehicle control, groups 3 had received distilled water (0.5 ml/kg, p.o.), group 4 was pre-treated with CoQ10 (10 mg/kg, p.o./day), group 5 received taurine (150 mg/kg, p.o./day), while group 6 was pre-treated the combination of CoQ10 (10 mg/kg, p.o./day) + taurine (150 mg/kg, p.o./day) However, from days 29 to 56, rats in groups 3-6 additionally received chlorpromazine (30mg/kg, p.o./day) once daily.  All treatments were made 30 min between each treatment. The rats were euthanized at the end of 56 days; Samples were collected for analysis. Biochemical parameters were measured by spectrophotometry. Data were analysed using ANOVA and differences in mean values were considered significant at p<0.05. Results: CPZ treated rats exhibited a significant decreased Na+/K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, H+-ATPase activities and sulphydryl content with corresponding increased in peroxynitrite formation as compared to control group.The reduction in Na+/K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, H+-ATPase activities by peroxynitrite formation may be through possible depletion of sulphydryl content. Decrease in sperm motility, immature sperm  and poor sperm function in CPZ can be attributed to these sulphydryl groups of the sperm membrane which plays a very important role in sperm motility and metabolism. Moreover, Pre-treatment with CoQ-10 (10 mg/kg, p.o) and taurine (150 mg/kg, p.o) significantly prevents the alteration observed in CPZ-treated rats. Conclusion: In conclusion, CPZ decreases sperm plasma membrane bound proton pump (ATPase) activities and inhibits sperm motiliy. It invokes nitrosative stress by possible mechanisms of peroxynitrite formation and depletion of free thiol content. The result from this study shows that CoQ-10 and taurine supplement has preventive effects on CPZ-induced peroxynitrite formation via its antioxidant and cyto-protectant activities. Hence, CoQ-10 and taurine could be use as therapeutic adjuvants for maintaining ion homeostasis necessary for sperm motility and maturation. Our results suggest potentially beneficial effects of CoQ-10 and taurine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.   Keywords: CoQ-10, taurine, CPZ, proton pump (ATPase) and sperm motility