Sports science is one of the newly exploring areas of Physiology that is very important to improve the athletic performance in scientific way. One of such a component that has a great probability to improve the athletic performance is the Antioxidant. More recently, Vitamin E as an antioxidant has come into the picture of muscular activity. Vit. E plays a critical role in protecting the cell from reactive oxygen species (ROS)-induced oxidative stress. There is an abundance of evidence that tissue content of Vit. E is decreased as a result of exercise, narrowing its protective margin against ROS. Because, human cannot synthesize this antioxidant Vit. E, they are dependent exclusively on dietary intake. So, oral supplementation of Vit. E may be a factor that influences physical performances. Total sedentary females were divided into two groups at random. Placebo sedentary group (n=15, given placebo supplementation for one week). Vitamin E-sedentary group (n=15, given 400 mg of Vit. E capsule supplementation for one week). Total female athletes were divided into two groups at random. Placebo athletes group (n=15, given placebo supplementation for one week). Vitamin E – athletes group (n=15, given 400 mg of Vit. E capsule supplementation for one week). Endurance performance has been carried out by Magnetic brake bicycle Ergometer on separate days. The room temperature was between 30-32 degree Celsius and the relative humidity was about 72%. The present study was to find out whether any physiological changes occur after Vit. E supplementation in female athletic and non athletic females. They are: 1. a. To investigate protective effect of Vit. E on lipid peroxidation as marker of oxidative damage during exercise. b. To evaluate the effect of endurance exercise and Vit. E supplementation on antioxidant enzyme systems in female athletes and sedentary females. 2. To study, at what extent an additional Vit. E intake can improve the cardio-respiratory parameters of athletes and non athlete females. On analyzing the data, it was observed that Vit. E, the fat soluble natural antioxidant, plays a critical role in protecting the cell from exercise induced free radical mediated oxidative stress. So,Vit. E supplementation (400mg) can be recommended to female athletes performing regular training and also to individual subjected to strenuous work. This study supports the idea of antioxidant effect of Vit. E which might be useful in the biological preparation of endurance athletes for improving their athletic performance.