Laser Doppler Flowmetry (LDF) is a well-known noninvasive technique for blood flow quantification. It provides a complex signal comprising several components at characteristic frequency ranges. These are related to the heart (0.6-2Hz), respiration (0.15-0.6Hz), myogenic wall (0.052-0.15Hz), sympathetic (0.021-0.052Hz) and endothelial NO-mediated (0.0095-0.021Hz) activities. Wavelet analysis is a suitable tool for the partition of non-stationary signals, as is the case of LDF. Our aim was to assess the responses in the lower limb skin microcirculation evoked when applying two different perfusion restriction protocols (protocol I – passive leg elevation at 45° from the supine position for 10 min; protocol II – suprasystolic occlusion at the ankle level with a pneumatic cuff in the sitting position for 3 min). Each protocol consisted of three phases: phase I – resting; phase II – provocation; phase III – recovery. A group of 30 healthy subjects (22,3 ± 3,7 years old) with 15 males and 15 females, given previously informed consent was studied. All procedures respected all the ethical standards for human research by the Declaration of Helsinki and subsequent amendments. Local blood flow, (AU’s) was assessed by the Periflux PF 5010 system (Perimed, Sweden) , probe placed in the inferior side of the second toe. The LDF signal, sampled at 32Hz, was partitioned using a Morlet wavelet transform (MATLAB). Amplitudes of these LDF components were compared for each phase in each protocol by the Wilcoxon matched-pairs signed-rank test. Variables were compared by protocol using the independent-samples Mann-Whitney U test. A 95% confidence interval was adopted. Registered amplitudes in protocol I were significantly lower than the ones registered with protocol II, which probably results from the blood distribution in the two anatomical positions. Protocol I results show that all amplitudes are significantly reduced during passive leg elevation. During recovery, only the cardiac and endothelial components failed to return to baseline, which suggests that leg elevation had a prolonged effect on the cardiac activity and endothelial NO synthesis. Protocol II results show that all activities are significantly reduced during occlusion, except for the sympathetic activity. During recovery, all components returned to baseline. These findings confirm the interest and usefulness of wavelet transform’s to look further into the in vivo regulation of circulation physiology.