We recently discovered that the well described singular urinary or Bowmans space within the Bowmans capsule could be split into three urinary spaces on an ultrastructural basis (Neal, 2003, 2005). Some of the fluid which filters across the glomerular filtration barrier (GFB) must enter a restrictive space under the podocyte (subpodocyte space, SPS) before entering the other urinary spaces. An anastomosing system of channels between the podocytes or interpodocyte spaces (IPS) are positioned in the glomerulus to drain filtered fluid from the centre to the edge of the glomerular capillary tuft and then into the shell like peripheral urinary space (PUS). The peripheral urinary space is very like the originally defined Bowmans space of 1842. Although we have measured, defined and described the subpodocyte and the interpodocyte spaces using reconstruction from ultrathin serial sections (Neal, 2005) we have not shown fully rotatable 3-dimensional reconstructions of the inter-relationships between the urinary spaces the podocytes and the glomerular capillaries, which we demonstrate here. Kidneys from humanely killed rats were either fixed with glutaraldehyde by immersion (n=3, renal arterial pressure of 0mmHg) or perfusion after a flush of the kidneys with Ringer solution (n=5, renal arterial pressure of 100mmHg throughout perfusion). Kidney pieces were postfixed, dehydrated, embedded and serial ultrathin sections of glomeruli were cut (1-2 glomeruli from each rat). Regions of the capillary tuft were reconstructed from electron micrographs using Reconstruct software (version 1.0.3.9 John C Fiala). The reconstructions show the close apposition between the podocytes and the glomerular capillary barrier. They clearly show the subpodocyte space and the anchoring processes which tether the podocyte to the glomerular basement membrane. With suitable highlighting the much more substantial anchoring processes of the perfusion fixed glomeruli can be seen in comparison with the immersion fixed glomeruli. Within the confines of the subpodocyte spaces the routes for movement of filtered fluid can be clearly seen along with exits from the SPS and routes through the interpodocyte and peripheral urinary spaces.