Aerobic capacity (V̇O2max) consistently manifests as the strongest predictor of future morbidity and mortality, superseding other more widely recognised risk factors such as hypertension (1). We recently collected data from 11 inactive individuals which suggested that V̇O2max can be improved with as little as two minutes of all-out sprint exercise per week (2); however, this finding requires confirmation in a larger cohort. In the present study, thirty-four sedentary but otherwise healthy men (n=16; age: 33±9 y; BMI: 25.1±2.1 kg∙m-2; V̇O2max: 39.2±8.6 ml∙kg-1∙min-1) and women (n=18; mean±SD; age: 36±9; BMI: 24.0±3.5 kg∙m-2; V̇O2max: 31.7±4.6 ml∙kg-1∙min-1) performed 18 supervised training sessions over 6 weeks on a cycle ergometer. The 10-min exercise sessions consisted of unloaded pedalling and one (first session) or two (all other sessions) brief ‘all-out’ sprints against a resistance of 5% body mass (10 s in week 1, 15 s in weeks 2-3 and 20 s in the final 3 weeks). V̇O2max was assessed during an incremental ramp test to volitional exhaustion (Lode Excalibur Sport, Groningen, the Netherlands) with continuous breath-by-breath measurements of V̇O2 taken using an online metabolic cart (ParvoMedics TrueOne 2400, Utah, USA). V̇O2max was taken as the highest 15-breath rolling average value achieved during the test. In all tests two or more of the following criteria were met: a plateau in V̇O2 despite increasing intensity, RER > 1.15, heart rate within 10 beats of age-predicted maximum, and/or volitional exhaustion. Despite the low total training time commitment (30 min/week of which no more than 2 min were at high intensity) and relatively low ratings of perceived exertion (RPE 14±2), two-way ANOVA revealed an increase in V̇O2max in both men (+9%; mean±SD: 3.22±0.50 vs 2.95±0.56 l∙min-1, p<0.01) and women (+10%; mean±SD: 2.28±0.36 vs 2.08±0.30 l∙min-1, p<0.01) following training, with no difference between sexes. In conclusion, herein we confirm that two minutes of all-out sprint exercise per week is sufficient to increase aerobic capacity in previously sedentary men and women.