We observed the adsorption process of myosin to the gold surface by QCM (quartz crystal microbalance). Using the AFFINIXQN Pro (Initium, Tokyo), viscoelasticity of the myosin adsorbed to the surface of the gold electrode and its surrounding solution as a whole was estimated by the response of quartz crystal to the resonance frequency and the frequencies in the vicinity. Samples were measured in both solution and air. The adsorbed protein volume was calculated from the data in the air. When myosin adsorbed more sparsely than 0.2 ug/cm2, viscoelastic change accompanied with myosin adsorption was almost the same as to the viscoelasticity of buffer except myosin. The resonance frequency falls as to weight of adsorbed myosin. This implies that myosin adsorbed at low density plays as a solid globular protein. On the other hand, when myosin adsorbed at higher density, large viscoelastic change has been obserbed. Viscoelastic analysis indicate myosin plays as a protein having viscoelasticity.
37th Congress of IUPS (Birmingham, UK) (2013) Proc 37th IUPS, PCD250
Poster Communications: Viscoelastic analysis of myosin adsorbed to gold
T. Ohno1, M. Wagatsuma2, M. Ichihashi2, A. Itoh2
1. Physiology, Jikei Univ., Tokyo, Japan. 2. Research & Development Division, Ulvac, Inc, Tokyo, Japan.
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