Objectives: Vitamin D has been found recently to play a role in respect to systemic diseases such as cardiovascular disease, cancer, and periodontal disease. We aimed to identify prevalence of vitamin D insufficiency and or deficiency in patients suffering from chronic periodontal disease and to analyze association between vitamin d status and chronic periodontitis severity.Material and Methods: In this cross-sectional study, we included women aged between 40 to 60 years with chronic periodontitis selected from the Counseling Center and Dental Care (CCDC) in Dental faculty of Rabat. Plasma baseline 25-hydroxy-vit D levels were measured byHigh Performance Liquid Chromatography Mass Spectrometry (HPLC-MS-MS)method. Deficiency was defined as ≤ 10 ng/mL 25 (OH) vitamin D and insufficiency as >10 and ≤30 ng/mL.Chronic periodontitis features (Clinical attachment level (CAL), gingival bleeding index (GI), and probing depth (PD) and Simplified Oral Hygiene Index ( OHI-S)) were determined in all participants by an experimented examiner. The severity of chronic periodontitis was defined according to the World Health Organization (WHO) classification. A statistical analysis was conducted to determine the relationship between chronic periodontitis and the status of vitamin D.Results: Eighteen patients (mean age of 48 ± 5.5 years [40-58]) were included until now. Abnormal status of vitamin D (<30ng/ml) was found in 88.9% of them (N= 16). There was no association between vitamin D levels and severity parameters of chronic periodontitis.Conclusion: Although preliminary due to the small sample size, results of this study suggest that Vitamin D deficiency was frequent in patients with chronic periodontitis. It may be convenient to look for vitamin D deficiency and to correct vitamin D nutritional status in chronic periodontitis patients. A case-control study on larger sample is ongoing to analyze association between vitamin D status and periodontal disease features.