Zingiber officinale has been documented to improve fertility while nicotine administration is associated with decline in sperm function and hormonal imbalances (Oyeyipo et al, 2013). However, the effect of Zingiber officinale on sperm function in nicotine treated male rats is still unknown. This present study was designed to investigate the effect of Zingiber officinale on nicotine-induced adverse effect on sperm function in male albino rats. Thirty two male albino rats weighing between 200-220g (8-10 weeks) were randomly divided into 4 groups (n=8) and treated orally for 30 days. Group I which served as the control received 0.2mg/kg normal saline, Group II received 1.0mg/kg BW of nicotine, Group III received 1.0 mg/ kg BW of nicotine alongside with 500mg/kg BW/day of aqueous extract of Zingiber officinale and Group IV received 500mg/kg BW/day of aqueous extract of Zingiber officinale. At the end of the experimental period, animals were anaesthetized with 1.9% ether and blood was collected through cardiac puncture. Serum was analysed for testosterone. Semen analysis showing sperm count, morphology, motility, volume and viability was also carried out. Values were expressed as mean ± S.E.M compared by ANOVA. Results showed that nicotine administration significantly decreased (p<0.05) testosterone in the 1.0mg/kg nicotine-treated group (2.92±0.34 ng/ml) when compared with the control (5.71±0.26 ng/ml). Co-administration of 1.0mg/kg nicotine with Zingiber officinale significantly increased testosterone (4.53±0.24 ng/ml) when compared with 1.0mg/kg nicotine-treated group There was significant decrease in sperm count (64.20±5.60 106/ml), motility (32.00±4.65 %) and viability (79.80±5.17%) in 1.0mg/kg nicotine-treated group when compared with the control (112.40±10.40 106/ml; 85.50±3.21%; 94.56 ±4.21%) respectively while nicotine-treated group co-administered with Zingiber officinale had significant increase when compared with 1.0mg/kg nicotine-treated group (108.30±8.14 106/ml; 83.80±5.01%; 90.60 ±3.38%) respectively. This finding shows that aqueous extract of Zingiber officinale ameliorates nicotine-induced decline in sperm function during nicotine administration attributed to the androgenic properties of the plant.