Objective: Previous study has showed that acupuncture analgesia is closely related to the functional status and degranulation of mast cells, and the analgesic effects of manual acupuncture rely on mast cell and its degranulation more. The present study recruited a c-kit gene knockout rats which mast cells are not expressed to explore the analgesic effects of manual acupuncture, different intensities of electroacupuncture- and moxibustion-like stimulation. Methods: The present study was performed on WsWs–, a c-kit gene knockout rat which mast cells is not expressed, and homogenous wildtype WsRC++ rats, twenty of both kinds were recruited to be control. The thresholds to noxious thermal and mechanical stimuli were measured with Ugo Basile Tail Flick Unit and electronic Von Frey anesthesiometer respectively. Somatic stimulation as 43 and 46 degree centigrade warm water, electroacupuncture of 1mA, 3mA and manual acupuncture were selected before pain thresholds tested. Results: For the WsRC++ rats, electroacupuncture, manual acupuncture and moxibustion increased the paw mechanical and thermal withdrawal latency, and high intensity stimulation showed better effect than low intensity stimulation, especially for electroacupuncture with high intensity. Compared in the WsWs–rats, the mechanical threshold increase induced by manual acupuncture in WsRC ++ was not attenuated, whereas the increased mechanical thresholds induced by electroacupuncture with low intensity and moxibustion were lower in knockout animals than those in wildtype, if not, even no increase was observed. Thermal threshold in c-kit knockout rats was not significant different from that of wildtype. Conclusion: For mechanical threshold, the analgesic effect of electroacupuncture might be closely related to mast cells. Whereas, the analgesic effect of manual acupuncture is not dependant on mast cells. And for the thermal threshold, none of the three stimulations was dependant on mast cells obviously.