Background and aims: We believe that GLUT2 (Glucose transporter 2) can be involved in the development of nonalcoholic steatohepatitis (NASH). Considering the importance of knowing the glucose flux in liver of obesity animals with nonalcoholic steatohepatitis, we studied the expression of GLUT2 protein. Materials and methods: We studied obesity mices (MSG) and control mices (C). The obesity was induced by neonatal subcutaneous dose of monosodium glutamate (2mg/g) during 5 days and a higher dose (4mg/g) at the 7th day of life. After the weanig, the animals were fed with normal fat diet (ND) and high fat diet (HD – 60% of fat) by 12 weeks. We obtain four groups: MSG-ND, MSG-HD, C-ND and C-HD. The obesity it not always related with the high weigh, thus measures of adiposity, Lee index and weigh of periepididymal adipose should be used. The GLUT2 protein was analysed by western blot. Paraffin-embedded liver were stained by regular hematoxylin-eosin (HE) methot for evaluation of liver histology. Results: The histological analyses demonstrated that nonalcoholic steatohepatitis is present in MSG-HD mices. In comparison to C-ND (P<0,05) and MSG-ND (P<0,01) animals, the MSG-HD showed higher weigh. The MSG-HD mices also showed higher Lee index when compared with the other groups (P<0,01). The weight of periepididymal adipose was higher in MSG-HD group when compared to C-HD group (P<0,01). The histological analyses shows nonalcoholic steatohepatitis in MSG-HD mices. In comparison to C-DN and C-DH animals, MSG-DN and MSG-DH mices showed increased GLUT2 protein content in and liver (P<0,05). Conclusions: We demonstrated a new data in the literature: the treatment of monosodium glutamate associated with high fat diet, leads to NASH. NASH is related to insulin resistance where there is a higher output of fatty acids and glucose to the liver. This glucose can be converted into triglycerides by the de novo lipogenesis, contributing for the hepatic steatosis. Thus, the GLUT2 can collaborate to NASH, by falicilitate the glucose input to hepatocyte
37th Congress of IUPS (Birmingham, UK) (2013) Proc 37th IUPS, PCC289
Poster Communications: Overexpression of GLUT2 protein in obesity mices with nonalcoholic steatohetatitis
A. D. Silva1, R. R. Favaro2, R. C. Petroni3, D. T. Furuya1, P. E. Silva1, H. S. Freitas1, A. C. Poletto1, M. M. Okamoto1, F. Barrence2, T. M. Zorn2, U. F. Machado1
1. Physiology and Biophysics, Institute of Biomedical Sciences - University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. 2. Cell biology and development, Institute of Biomedical Sciences - University of Sao Paulo, Sao Paulo, Brazil. 3. Medical Sciences, Medical School - University of Sao Paulo, Sao Paulo, Brazil.
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Where applicable, experiments conform with Society ethical requirements.