A tremendous amount has been learned over the last decade regarding the ionic and molecular basis of cardiac regional electrical specialization. Regional variations of ion channel subunits in specific cellular subtypes in complex regions of the left ventricle remained largely unknown. There is no debate over the importance of these ion channels in the normal functioning of the heart and in arrhythmogenesis. Therefore, the aim of the present study was to explore the ion channel distribution of KvLQT, minK, ERG, NCX, Cx43, SERCA2a, Nav1.5 in 9 – 9 segments of the left ventricle epi- and endocardial wall. Hearts were isolated from adult male NZW rabbits (n=8-5, 2.0-2.5 kg) following pre-medication with medetomidine hydrochloride (0.2 mg/kg), ketamine (10 mg/kg), and butorphanol (0.05mg/kg) (sc.). Animals were sacrificed by an overdose of pentobarbitone sodium (111 mg/kg, iv.)(1). All procedures were undertaken in accordance with ethical guidelines set out by the UK ASPA. LV tissue samples were dissected 18x1mm2 pieces and were cleaned from free running Purkinje fibers, and Papillary muscles. Q-PCR was performed using SYBR green technology(2, 3). Experimental data points in triplicate were analysed using ΔCt method to compare the relative abundance of different transcripts with 28S, 18S or GAPDH. Statistical significant differences were accepted at p values of *p<0.05, **p<0.01 using ANOVA with Tukey’s Post hoc test. Using ΔCt method in the distribution of KvLQT, ERG, Nav1.5 and Cx43 genes there were tendency differences at a non-significant level. In SERCA2a and NCX there were no differences transmurally and through the base-apex axis. Using ΔΔCt method comparing minK expression level in base to apex or epi- to endocardium, there were no significant differences through 8 transmural and 5 base/apex sites. MinK was 2.019±0.25 times more abundant in the epi-, than in the endocardium in the mid posterior wall, and 1.77±0.31 times higher in the apex than the base in the anterior apical region, which is in contrary with our previous finding, where protein level of minK was lower in the apex than the base(1). Presumably this discrepancy can be attributable to differences in location in the LV. Most of the no differences are in line with ion channel distribution found in the neonate rabbit ventricle(2). In previous findings it has been shown(2) that KvLQT, ERG, Nav1.5, minK expression level decreased transmurally in basal free wall of the adult rabbit LV. Reason for the different finding can be amongst lots of other criteria the different tissue collection method. However, the aim of the present study was to clarify the previous findings and give a deeper understanding of the transmural and base-apex differences in ion channel distribution, it still remained incompletely understood. Further studies are planned for reaching success.