Background: Nitric Oxide (NO), a signalling molecule and potent vasodilator linked to improved vascular function is derived from the catabolism of L-Arginine (1), and the reduction of Nitrate (NO3-) and Nitrite (NO2-) (2). The ingestion of dietary nitrate has been reported to improve exercise performance with a lower oxygen cost evident at submaximal work rates (3). Few studies have investigated the effect of dietary nitrate ingestion on short duration supra-maximal exercise performance. Methods: Eight trained cyclists participated in a double-blind randomised cross-over study. A single 140 ml dose of concentrated beetroot juice (8 mmol NO3-) or placebo (concentrated blackcurrant juice) was ingested 2 hours prior to exercise. Participants completed five 10 s all out sprints interspersed with 30 s recovery, on a cycle ergometer. Peak and Mean power outputs (W) were recorded for all sprints. Following conformation of distribution normality (Shapiro-W-Wilk Tests), data were analysed using repeated-measures ANOVA. Wilcoxon signed rank order tests were used for post hoc analysis. Significance was established at P < 0.05 and data are expressed as mean ± SD. Results: Peak power improved for all sprints under nitrate supplemented conditions, however only sprints one (a 3% improvement from 1144W to 1180W) and three (a 6% improvement from 872W to 933W) increased significantly (P < 0.05). Mean sprint power trended towards improved power outputs under nitrate supplemented conditions albeit without statistical significance. Conclusions: These findings suggest a possible ergogenic effect of dietary nitrate ingestion on short duration supra-maximal repeated sprint exercise. Further research is required to confirm these findings and investigate potential mechanistic bases.