Cigarette smoking is worrisome in adults and also in adolescents. Actually, smoking rates have grown and little is known about the damages caused in these organisms still young and the consequences in adulthood (1, 2). This study aims evaluate the influence of smoking in puberty and the consequences of withdrawal in body composition of female and male mice. At 35 days, Swiss mice reproduced and maintained at the animal house of Phisiological Sciences department were exposed to 3R4F cigarette smoke (Tobacco and Health Research Institute), 8 h/day for 15 days (S, n = 24).Then, half of animals were evaluated and the other half was maintained and evaluated thirty days after stop exposure (30d AS). An unexposed group accompanied the events (NS, n = 24). Body mass, food intake, body composition (by Dual-energy X-ray Absorptiometry, DEXA), serum levels of insulin, lipids and glucose (ipGTT), subcutaneous and intra-abdominal adipose tissue mass were evaluated. During all experimental period food intake was similar in all groups (5.06±0.35g), however, S groups showed lower body mass gain. 30dAS, female S group presented similar body mass (36.39±0.56g) while male S group presented lower body mass (NS: 46.64±1.05g; S: 45.06±1.05g). In females was observed a significant increase in total body (NS: 11.86±0.07g; S: 15.17±0.87g) and trunk fat (NS: 9.17±1.16g; S:13.00±0.68g) during exposure and in lean mass 30dAS (NS: 13.60±1.89g; S:17.25±1.49g), while no alterations were developed in male’s body composition. In males none region of fat differs during exposure: retroperitoneal (NS:0.411±0.030; S:0.475±0.079), mesenteric (NS:0.344±0.036; S:0.349±0.051) and subcutaneous (NS: 0.243±0.066; S:0.290±0.062). While in females the retroperitoneal was increased (NS: 0.360±0.039; S:0.575±0.079), the mesenteric decreased (NS:1.077±0.08; S:0.917±0.242) and subcutaneous did not alters (NS: 0.217±0.021; S:0.287±0.026). In regard to serum analyses during exposure, females had higher insulinemia and glycemia at 30min but no alterations were observed in males. On the other hand, withdrawal induced in male high insulinemia and low glycemia but in female, normal insulinemia and low glycemia. The set of results indicates different response to cigarette smoke in young that seem to start in females and lead malefic metabolic alterations in adulthood.