In vitro neurite outgrowth analysis by fluorescence microscopy is an important tool for studies in the fields of neurodegenerative diseases such as Alzheimer’s and Parkinson’s, neuroregenerative processes such as spinal cord injury repair, and basic neurological research. Manual measurement of outgrowth is a time consuming process and potentially biased process. Using the Neurite Outgrowth application module of the MetaMorph® 7.0 software package we analyzed two dimensional fluorescent images of PC12 cells in vitro, stimulated with NGF, and compared the results with those using manual measurements. Variances and correlations are shown between multiple users, multiple measurements by the same user, and software analysis of replicate experimental samples. These results demonstrate that automated analysis can provide results faster and more reproducibly and statistically similar to traditional manual methods.
Life Sciences 2007 (2007) Proc Life Sciences, PC99
Poster Communications: Automated Neurite Outgrowth image analysis – validation versus manual measurement
P. Djali1, M. David1, J. Bosworth1, N. Gliksman1, S. Handran1
1. MetaMorh Imaging, Molecular Devices Ltd, Wokingham, United Kingdom.
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Where applicable, experiments conform with Society ethical requirements.