Integrin β4 was found downregulated on the airway epithelial cell of asthma patients which induce the damaged anti-oxidation ability, inhibited wound repair ability and suppressed Th1 differentiation after OVA stress. These results strongly suggested that decreased expression of integrin β4 may relate to asthma susceptibility. To verify this correlation, in this paper, we construct a model that integrin β4 was conditional knocked out by Tetracycline. Integrin β4 floxed heterozygous mice have been constructed by transgene technology. Cre-CCSP-(otet) double transgenic mice was constructed by double crossing CCSP-rtTA line and (otet)-Cre line. Then floxed integrin β4 Cre-CCSP-(otet) triple transgenic mice was comstructed by crossing Cre-CCSP-(otet) line and integrin β4 floxed mice. By RT-PCR and immunohistochemistry detection, our results showed that integrin β4 expression was deleted successfully after Tetracycline induction in floxed integrin β4 Cre-CCSP-(otet) triple transgenic mice.
37th Congress of IUPS (Birmingham, UK) (2013) Proc 37th IUPS, PCC064
Poster Communications: Construction of asthma susceptivity model by conditional deletion of integrin beta 4 on airway epithelial cell
C. Liu1, X. Qin1, H. Liu1, Y. Xiang1, X. Qu1, Y. Wang1
1. Central South University, Changsha, China.
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