Formamide-induced detubulation of ventricular myocytes isolated from the hearts of rats, which were humanely killed using a Schedule 1 procedure, will be demonstrated. This technique was described in the talk ‘Subcellular heterogeneity in ventricular myocytes’ presented in the symposium ‘Cardiac Electrophysiology: Heterogeneity in the Heart’ at this meeting (Orchard et al. 2002) and used in the study described by Brette et al. (2002).
This work is supported by The Wellcome Trust and the British Heart Foundation.
All procedures accord with current UK legislation.