Objective: Changes in skin microcirculatory blood flow assessed by laser Doppler flowmetry (LDF) represent well systemic microvascular function (1-3). Oxidative stress appears to be the common underlying cellular mechanism for the development of endothelial dysfunction with subsequent changes in microvascular reactivity in various vascular diseases (4,5). The aim of this study was to evaluate the effect of oxidative stress on skin microcirculatory blood flow in young healthy individuals. Materials and Methods: 40 healthy female medical students (age 20-23 yrs), volunteered to participate in this study. The study protocol and procedures conformed to the standards set by the latest revision of the Declaration of Helsinki and were approved by the Ethical Committee of the Faculty of Medicine, University of Osijek. Skin microcirculatory blood flow was assessed by LDF (MoorVMS-LDF, Axminster, UK). Changes in blood flow were measured during baseline and reactive hyperaemia following release of a 1 minute occlusion of blood flow Lipid peroxidation was measured by TBARS method (Thiobarbituric Acid Reactive Substances) and antioxidant activity by FRAP (Ferric Reducing Antioxidant Power) method in venous blood samples taken before the occlusion. Correlation analysis was calculated using Pearson’s correlation coefficient at 95% confidence of interval (SigmaPlot v11.2, Systat Software, Chicago, USA). Results: There was statistically significant negative correlation between reactive hyperaemia in skin microcirculation and lipid peroxidation (R and TBARS, Pearson r = -0.3309, R2=0.1095, P=0.037). There was no correlation between reactive hyperaemia and plasma antioxidant activity (FRAP) (R and FRAP, Pearson r=0.1404, R2=0.01973, P=0.3874). Conclusion: The results of this study clearly suggest that the level of common oxidative stress has significant effect on microvascular reactivity in the population of young healthy individuals, and that increased oxidative stress may affect normal vascular function. This study shows that the maintaining of oxidative balance has an important role in preservation of vascular health.