The global rises in the prevalence of obesity, diabetes mellitus and associated diseases, led to an increase of sweeteners consumption around the world. The presence of nutrients in the small intestine plays important roles in appetite regulation, blood glucose (BG) and gastric emptying (GE). These regulatory roles are mediated by peptides called incretins, such as glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP). The release of these peptides is stimulated mainly by nutrients, whereas the effects of sweeteners, in vivo, on incretins release are not well established. Objective: To determine the effects of addition of sucrose, sorbitol, sucralose or stevia on a standard liquid test meal, on GE, on glucose homeostasis (BG and plasma insulin), as well as on GIP and GLP-1 release in humans. Design: Eleven male healthy volunteers were studied on 4 separate occasions. GE of a caloric test meal (205 kcal), containing technetium (99mTc), were determined by abdominal scintigraphy. Fifteen grams of sucrose, sorbitol, sucralose or stevia were added to the test meal, one each occasion. The GE of test meal was measured by T1/2. Blood glucose, plasma insulin and incretins were determined at t = -10 (fasting), 0, 5, 10, 15, 30, 45, 60, 90 and 120 min after the meal ingestion using a glucometer and specific immunoassay kits. Results: GE of a standard test meal prepared with sucrose was faster than when sorbitol or sucralose were added. The addition of sucrose or sweeteners in the standard meal, did not cause significant difference of BG. The insulin concentrations were significantly raised after the ingestion of sucrose as compared with sorbitol or stevia.The addition of sucrose, sorbitol, sucralose and stevia increased the GLP-1 plasma concentration equally. This increase was different, between sucrose and sucralose, only 120 min after the meal ingestion. Regarding GIP, the meal prepared with sucrose was able to increase its concentration compared with the different sweeteners. Conclusions: Our results showed that the consumption of sweeteners like sorbitol or sucralose, added in a balanced test meal (composed by carbohydrates, protein and lipids), were able to delay the GE. Moreover, the consumption of these substances added in a balanced meal, did not change the incretins release as well as the glucose homeostasis in healthy humans.