Change in lifestyle, as increased consumption of dietary fats and reduced physical activity in modern society, are causes of obesity that can lead to diabetes melliuts, hypertension and dementia. Maternal obesity has been documented to play a direct role in the transmission of obesogenic and diabetogenic traits to the next generation and has also been shown to influence the nervous system of offspring. A high-fat diet (HFD) disrupts cognition and contributes to neurodegenerative diseases as well as impairing hippocampal synaptic plasticity, neurogenesis and cognitive abilities. The aim of these study was investigate the effects of maternal high-fat intake in the hippocampal formation of offspring at 10 days of age. Female adult Wistar rats were feeding with a normal diet (ND, 4% soybean oil, n=6) or a high-fat diet (HFD, 19% soybean oil, n=7) during pregnancy and lactation. Mother and offspring body mass and food intake were monitored. Lipid profile was evaluated. Intraabdominal adipose tissue were collected and weighted. For morphology evaluation, the rats were anesthetized and perfused intracardially with 4% paraformaldehyde in 0.1M PBS (pH 7.4). The brains were transferred to a 30% sucrose solution and then cryoprotected and sectioned serially at 20 µm. The imunohistochemistry evaluation used Ki-67, for cellular proliferation associated with identification of Neu-N (Millipore) – a marker for mature neurons or GFAP (Sigma), a marker of astrocytes. In pregnancy the average body mass of the rats was 234.2 ± 10.91 g. However, at the end of the lactation period the rats of HFD group showed 26% of gain in body mass, without significant difference of food consumption. Offspring of mother fed with HFD showed an increase (16%) in body mass and triglycerides, but intra abdominal adipose tissue mass did not differ. Labeling with Neu-N demonstrates that the granular layer appears thicker in both GD as Ammon’s horn (CA1 and CA3). The double labelling (Ki-67 /GFAP) suggests an increase of proliferative cells in the dendate gyrus. The staining with anti-GFAP was more intense and presents more radial profiles (radial glia), suggesting an increased proliferation and/or developmental differentiation of astroglia. Thus, it seems that maternal diet can affect metabolic parameters and slow hippocampal development.