Objective: To assess the impact of birth order on child development among preschool children. Design and method: A cross sectional study was conducted through anganwadi schools among 530 children at three years staying in a rural community in a Primary health centre area, to assess the developmental delay using screening tool, Ages and stages questionnaire (ASQ). Then, these children were correlated and analysed for birth order in the family with their global development. The study was approved by Institutional Ethics Committee for Human Subjects Research. Chi-square test was used to compare categorical variables. Differences were considered significant at P<0.05 level. Results and conclusions: The prevalence of global developmental delay assessed by ASQ was 19.8%. [1] A majority of the children (85.8%) were from joint family and majority (81.5%) were of 1st and 2nd birth order. In the present study, ASQ score for personal social skill was low for a child who stayed with fewer number of children at home as against those who stayed with more number of children (P=0.07), though not statistically significant. The gross motor development was better in 1st and 2nd order children compared to higher order and this showed a statistically significant change (P=0.04). The rest of the domains didnot show any significant correlation. The results of the present study showed that there was a trend towards higher ASQ scores from higher birth order to lower birth order.