Adipose tissue is a source of inflammatory cytokines, such as TNF-α, characterizing a chronic low-grade inflammatory state (1). Lifestyle behavioral interventions, as physical activity, may have clinically significant benefits for improving inflammation (2). We aimed to investigate inflammatory responses in muscle after intermittent training of high intensity squat jump type in Wistar obese-induced rats. Forty adults male Wistar rats of 2 months-old were divided into: sedentary control (SC), exercise control (EC), sedentary obese (SO), exercise obese (EO). Control groups were fed with standard chow and obese received a cafeteria diet+standard chow ad libitum. The training protocol was performed following the adapted model of force from Tamaki et al. (3). It consisted on 3x a week, interposed by 24h among sessions, 3 series of 12 repetitions of 60s of interval among series with a strength load equivalent to 50% of the body weight (BW) during 6 weeks. An insulin tolerance test (ITT) was performed with venous blood collected from tail distal end for glicemia determinaton after ip injection of regular human insulin (0.5 IU/kg BW). The sacrifice was performed for removal of soleus muscle under anesthesia (Ketamine 80mg/kg,Xylazine 5mg/kg,ip). RT-PCR was performed for evaluation of Tnf and Il10 mRNA levels. ANOVA one-way was used for comparison among means with post-hoc (Tukey) if necessary, considering p<0.05. The experimental protocol was approved by the Ethics Committee for Animal Research (#15/2009). Obese (SO=538+/-19.6, EO=494.6+/-14; p<0.01vsSC,EC) presented a final weight significantly higher than controls (SC=415+/-18, EC=393+/-14) and significant increase in fat mass (around 49% in SO, p<0.001 vs SC; 42% in EO group p<0.01vsEC). ITT showed a reduction in insulin sensitivity in obese, however reversed by training (kITT expressed as %/min: SC=5.76+/-0,36;EC=6.63+/-0.45;SO=3.60+/-0.62*#; EO=7.07+/-0.47&;*p<0.05vsSC;#p<0.01vsEC, &p<0.001vsSO). The diet increased fat mass (SC=10.6+/-0.91,SO=20.83+/-0.17×0.001g/g BW, *p<0.001), and Tnf RNAm levels in obese group (SC=100.5+/-5.3, OS=133+/-7.9 AU, p<0.001). The training caused a 27%-reduction (p<0.01vsSO) in fat mass, and 39%-reduction in Tnf mRNA level (EO=80.7+/-6.2, p<0.05vsSO). The cafeteria diet positively modulated, while the training reduced Il10 levels mRNA (SC=100.6+/-4.2&, SO=210.4+/-10, EC=42.8+/-9.5&#, EO=153.9+/-13.7*, *p<0.05vsOS, &p<0.001vsSO, #p<0.01vsEO). We can conclude that the cafeteria diet was detrimental to insulin sensitivity in rats, and effective in body weight gain and increase of inflammatory cytokine TNFα levels. On the other hand, high-intensity intermittent training was effective in reversing this situation. Surprisingly, high-intensity intermittent training worsened, but the diet per se increased IL10 gene expression.