Interleukin-1β (IL-1β) and -6 (IL-6) are inflammatory cytokines that are involved in bone resorption under pathological conditions. The cytokines are also involved in bone remodeling under physiological conditions. Voltage sensitive Ca2+ channels (VSCCs) serve as crucial mediators of membrane excitability and many Ca2+-dependent functions such as growth of bone, regulate proliferation, differentiation, enzyme activity and gene expression. The effects of IL-1β and -6 on VSCCs in osteoblast cell line (MC3T3-E1) were investigated using patch-clamp recording. Our results showed that application of 50 pM-50 nM IL-1β facilitated VSCCs current (ICa) carried by Ba2+ (IBa). Application of 50 pM-5 nM IL-6 facilitated IBa. In contrast, 50 nM IL-6 inhibited IBa in MC3T3-E1 cells.
37th Congress of IUPS (Birmingham, UK) (2013) Proc 37th IUPS, PCA323
Poster Communications: Interleukin-induced calcium channel current modulation in osteoblasts
H. Kobayashi2, T. Endoh1, M. Tazaki1, K. Sueishi2
1. Dept of Physiol, Tokyo Dental College, Chiba, Japan. 2. Department of Orthodontics, Tokyo Dental College, Chiba, Japan.
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