Previous studies have shown significant increases in intestinal glucose uptake (IGU) following hyperglycemia of any cause. It is not clear how the intestine would respond to other sugars apart from glucose. The present study therefore was designed to investigate the effects of intravanous (i.v) infusion of fructose, galactose and glucose on canine IGU during postprandial state. Experiments were carried out on fasted, male, anaesthethized adult mongrel dogs divided into four groups with 5 dogs per group. Each of the groups was given i.v infusion of normal saline, fructose (1.1mg/dl/min), galactose (1.1mg/dl/min) and glucose (1.1mg/dl/min) respectively. Through a midline laparatomy, the upper jejunum was secured and cannulated for blood flow measurement. Blood samples were obtained for measurement of glucose content of arterial and venous blood from the upper jejunal sgment.The blood glucose was determined by glucose oxidase method and intestinal glucose uptake was calculated as the product of jejunal blood flow and arterio-venous glucose difference. Values are means ± S.E.M, compared by ANOVA and Student t-test. Fructose, galactose and glucose significantly increased arterial blood glucose from 97.60±1.78 mg/dl to 114.20±1.88mg/dl, 109.80±1.43mg/dl, and 141.20±5.65 mg/dl respectively. Glucose also significantly increased jejunal blood flow from 10.0±0.32ml/min to 14.40±0.93ml/min, however, fructose and galactose did not produce any significant effect on intestinal blood flow. IGU increased by 600%, 350%, and 700% in response to fructose, galactose and glucose respectively. There is no correlation between the increase in blood glucose levels induced by each of the sugars and its corresponding rise in IGU. The data suggest that the intestine responds to fructose and galactose in a similar manner as glucose. However, the mechanism of the responses may not be the same in the three sugars.