Kolaviron (KV) a fraction of Garcinia kola has been found to have hypoglycemic effect on glucose metabolism. The mechanism of its hypoglycemic effect was investigated. KV was isolated according to Iwu et al(1990).The study was executed in three phases. Phase I was conducted to investigate the effect of KV on blood glucose concentration in rats (male, 180-200mg), n= ,6) fasted for 18 hours while phase II and IIIwere to investigate the route(s) of glucose utilization in KV treated rats via the artero-venous (A-V) and intestinal glucose absorption where 10-20 cm long segments of the jejunum and ileum were used respectively. The rats (male, 180-200mg) were grouped into four groups of six animals each. Group I represented the control(treated with ?saline) while group II , III and IV were treated with 50mg, 100mg and 200mg of KV per kilogrammme body weight respectively via oral administration using a metal feeding canular. The rats were anaestesized in phases II and III with urethane (0.6ml/ 100mg body weight) given subcutaneously prior to laparotomy. Values bare represented as Mean+/- S.E.M., compared by one way analysis of variance and unpaired Student’s ‘t ‘ test. Results from phase I showed significant decrease in blood glucose concentration from the various treated groups after 4 hours of administration, ( Control= 69.00+/-2.66 mg/dl , KV 50mg/kg= 64.33+/-2.40mg/dl, KV 100mg/kg= 59.83+/-2.48 mg/dl and KV200mg/kg= 54.00+/-2.82mg/dl) . The A-V study revealed a 13.7% , 36. 99% and 47.94% decrease in blood glucose concentration in the KV50, KV 100 and KV 200 mg /kg groups respectively compared to the control group after 3 hours of administration of KV. The jejunum and ileum glucose absorption significantly increased in all the treated groups compared bro the control animals after 40 minutes of infusion, P<0.05. KV possibly exerts its reported profound hypoglycemic effects through the increased glucose extractions by intestinal cells. More work is required to determine the possible role of insulin and counter- regulatory glucose hormones in mediating the hypoglycemic effect reported.