Potassium channels form the most diverse family of ion channels with more than 70 genes cloned in humans. They can be broadly subdivided into three structural classes made of two, four or six transmembrane segments (TMS) (Patel & Honoré, 2001a). All potassium channels can be recognized by the presence of a conserved motif called the P domain (the pore-forming region), which is part of the potassium conduction pathway. The two TMS and six TMS classes contain a single P domain while the class of four TMS subunits contains two P domains in tandem. Functional potassium channels are tetramers of pore-forming subunits for the two and six TMS classes and probably dimers in the case of the four TMS class.
The family of mammalian 4TMS/2P potassium channel subunits has increased to 14 members (Patel & Honoré, 2001a). These subunits share the same structural motif but low sequence identity is found outside the P domains. TREK-1 (KCNK2), TREK-2 (KCNK10) and TRAAK (KCNK4) subunits form a structural as well as a functional subgroup. Human TREK channels are highly expressed in the central and peripheral nervous systems, but are absent from the heart. TREK channels are opened by a variety of physical and chemical stimuli (Patel & Honoré, 2001a).
TREK channel activity is elicited by increasing mechanical pressure applied to the cell membrane (Patel & Honoré, 2001a; Patel et al. 2001). At the whole cell level, TREK-1 is modulated by cellular volume. Intracellular acidosis converts TREK mechano-gated channels into constitutively active channels. Finally, heat gradually and reversibly opens TREK-1 with an exceptional Q10 value of 7. Deletional analysis demonstrates that the carboxy terminus, but not the amino terminus and the extracellular M1P1 loop, is critical for activation of TREK-1 by stretch, intracellular acidosis and heat.
TREK channels are reversibly opened by anionic polyunsaturated fatty acids including arachidonic acid. The activation is either direct on the channel protein or via a membrane effect (Patel et al. 2001). Lysophospholipids containing large polar heads are additional potent openers. TREK channels are the target for volatile general anaesthetics including halothane and isoflurane (Patel & Honoré, 2001b). Opening of these channels by inhalational anaesthetics induces cell hyperpolarization and may contribute to general anaesthesia (Patel & Honoré, 2001b). Chemical activation of TREK-1 is also critically dependent on the carboxy terminal domain (Patel & Honoré, 2001a, b; Patel et al. 2001).
TREK channels are additionally regulated by the neurotransmitter/cAMP/PKA pathway (Patel & Honoré, 2001a). For instance, opening of TREK-1 by lipids is reversed by protein kinase A stimulation. Protein kinase A-mediated phosphorylation of Ser333 in the carboxy terminus mediates TREK-1 closing. TREK-1 activity is also inhibited by the protein kinase C pathway, although the phosphorylation site remains to be identified.
The biophysical and pharmacological properties of TREK channels resemble those of the Aplysia S-type potassium channel (Belardetti & Siegelbaum, 1988; Patel & Honoré, 2001a). The S channel is responsible for the control of presynaptic facilitation of transmitter release that underlies behavioural sensitization, a simple form of learning and memory. Considering that TREK channels are highly expressed in the hippocampus and the cerebral cortex, they may play a significant role in cognition (Patel & Honoré, 2001a).