This work have been supported by Dept. of Biology, School of Science, Shahid Chamran University, Ahvaz, I.R. of Iran.
University of Heidelberg (2006) Proc Physiol Soc 4, PC6
Poster Communications: Neda Dabir1, Ahmad Moazedi1, Mohamad GharibNaseri2, Mohamadreza Zadkarami1
1. Department of Biology, School of Science, Shahid Chamran University, Ahwaz, Iran. 2. Department of Physiology, School of Medicine, JondiShapur University, Ahwaz, Iran.
Rue (Ruta chalepensis) belongs to the Rutaceae family. It grows in the north of Boshehr(south Iran). The plant is rich in coumarins, it has anti-fertility effects and some quinoline alkaloid mutagenic actions as well as curare-like ganglionic–blocking effects and spasmolytic activities (1). The aerial part of Ruta chalepensis is used for delaying the onset of seizures; a dose-dependent suppression in the tonic phase induced by pentylenetetrazole (PTZ) has been reported (2). Its extract increases blood levels of nitrite, an indicator of nitric oxide production (3). This study investigated the effect of a hydroalcoholic extract of Rue leaves (RLHE) on the contraction of rat ileum induced by 60 mM KCl (4). Rue leaves (10 g) were macerated in 70% ethanol. The mixture was filtered and the solvent was evaporated. Ileum segments were removed post mortem from adult male Wistar rats (225±25 g) and placed under 1 g initial tension in an organ bath containing Tyrode solution (pH=7.4, 37║C). Contractions were recorded using an isotonic transducer and oscillograph. Statistical comparisons performed using Student's paired t test and one-way analysis of variance (ANOVA), show that various concentrations of Ruta chalepensis extract (0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07 mg/ml) significantly (n=7, P<0.001) decreased the KCl-induced contraction. Laboratory experiments were performed to investigate the mechanism of relaxation of the rue extract on smooth muscle with the β-adrenergic receptor antagonist propranolol (1 µM). Block of the β-adrenoceptors by propranolol did not significantly affect the relaxation response of rue. Since the present study showed that propranolol had no influence on the relaxation effect of smooth muscle ileum, it seems therefore that the relaxation effect of rue leaf extract was not performed by β-adrenergic receptors.
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