The Petrochemical Industry is based upon the production of chemicals from petroleum such as natural gas and crud oil. Hydrocarbons are known to be human carcinogens and are associated with the development of chronic health disorders. The aim of the present study was undertaken to investigate the oxidative stress, antioxidant defense response, liver and kidney function parameters and lipids profile and to assess the environmental safety awareness in Egyptian petrochemical workers (300 workers) who are exposed to hydrocarbon. The study was approved by the committee of ethics in research of the Alexandria University and informed consent was required from all participants, according to the guidelines of the local ethics committee. Fifty workers and thirty control subjects were enrolled in the present study. All subjects were matched for age, sex and socio-economic level. Venous blood samples from all the subjects were collected with the anticoagulant, EDTA. Results showed a significant increase in kidney and liver function parameters in addition to lipid proxidation. On the other hand, a significant decrease in enzymatic (SOD, CAT, GST) and non enzymatic (GSH) antioxidants was observed. Also, perturbations in some hematological parameters and lipids profile were detected. In conclusion, a state of oxidative stress in hydrocarbon exposed workers is produced by the depletion of antioxidant capacity that may play a role in the mechanism of its toxicity. Also, environmental safety requirement is good but need more application and update.