The WHO in 1997(5) stated that 12 gm/dl is the borderline for normal Hb level for adult and 15 as a normal mean (16 and 14 gm./dl for male and female respectively) .Dacie & Lewis stated Hb value of 13.0+1.5 gm./d for children 10-12 yl,(4).Few researchers in Sudan worked in reference Hb value, among them Abdella et al in 1987 (1) found the mean of Hb reference value of 12.00gm/dl for children between 6 to 12 y. with no sex difference. Atig in 1995 (3) stated Hb reference value of 11.63+ 0.89gm/dl for Sudanese children between 5 to 12 y. in Khartoum state. Agabeldour (2) in 1997 worked out Hb reference value of 13.18+ 1.46gm/dl for adult males and a value of 11.17 + 2.03 gm/dl for adult females in Sudan Objective:- The objective of this study is to estimate the reference Haemoglobin value in apparently healthy Sudanese children in Khartoum state, Sudan. To achieve this objective, using stratified random sampling, 1000, capillary blood samples were collected from apparently healthy Sudanese children in Khartoum state within the age group 12 – 14 years, 550 females and 450 males. The subjects were interviewed according to a designed questionnaire, clarifying tribe, nutritional and socioeconomic status as main variables, Haemoglobin (Hb) value was determined for each, using Hb-cyanide method. The mean reference Haemoglobin value for both sexes was 11.69g/dl+ 2.13 while in males and females was 11.67g/dl+ 2.13, and 11.70g/dl+ 2.13 respectively. About 50% of the children reported value less than 12g/dl, while 6.3% showed a value of 9.4g/dl or less, without any symptoms of anemia. Gender and income has no Significant effect on Haemoglobin value in this study, while nutrition and ethnic background affect Hb value, Southerners have the lowest Hb value, while Kordofan tribes have the highest. Conclusion :- the mean reference Haemoglobin value for both sexes within the age group 12 – 14 years in Sudan is considerably lower than the standard ones stated in books and, differ according to tribes