Stress through its response, be it chronic or acute, produces behavioural and chemical deficits that affect most body functions especially reproduction (Selye, 1936; Khodaei-Motlagh et al., 2011). Although chronic exposure to stress has been reported to affect reproduction in an adverse manner (Krulich et al., 1974; Blake, 1975; Hillier and Tetsuka, 1998), effects of acute exposure to stress on reproduction have been controversial. To study the effects of repeated acute restraint-induced stress on reproductive hormones in female rats, fifteen female Wistar rats were divided into three groups of five rats each. Groups I and II rats were restrained for 10 minutes and 20 minutes respectively for seven days while group III rats served as control. All the rats were weighed everyday before the restraint throughout the duration of the experiment. On the 7th day, immediately after the restraint, the rats were anaesthetized using chloroform and killed, then their blood samples collected into heparinized tubes and centrifuged. The supernatant plasma from each blood sample was then used to analyse reproductive hormones and cortisol. Statistical analysis was done with one-way ANOVA and the results were expressed using mean ± SEM. The results showed that there were increases in weights and hormonal levels of the restrained rats compared to the control. The weight increase was significant only in 10 minute restrained rats when weights of days 1 and 7 were compared (180 ± 9.08g vs. 188 ± 8.60g). In 10 minute restrained rats, levels of oestradiol (240 ± 17.55pg/ml), progesterone (41.25 ± 1.94ng/ml) and FSH (1.33 ± 0.06mIu/ml) significantly increased when compared to their corresponding control values of (174.00 ± 8.28pg/ml), (13.13 ± 1.40ng/ml) and (0.3 ± 0.3mIu/ml) respectively. Levels of cortisol (7.63 ± 0.76µg/ml), LH (4.80 ± 0.49mIu/ml) and prolactin (4.25 ± 0.32ng/ml) also increased in the 10 minute restrained rats compared to their control values of (3.90 ± 1.10µg/ml), (4.42 ± 0.28mIu/ml) and (3.50 ± 0.20ng/ml) respectively but the increases were not significant. Thus, it was concluded that acute stress such as 10-minute and 20-minute restraint used in this study could lead to increases in body weight and reproductive hormones.