Studies in humans and hypertension models have demonstrated the role of the RAS in the progression of ventricular dysfunction. The activation of the renin-angiotensin system (RAS) is essential for structural and functional remodeling of the heart. Additionally, it is recognized the role of sex hormones (SH) modulating the RAS. Therefore, we aimed to evaluate the influence of SH on the activity of angiotensin I-converting enzyme (ACE) and angiotensin 2-converting enzyme (ACE2) in male and female rats SHR and the influence of ACE inhibitor in the SRA local and plasma deficiency in HS. Materials and methods: Female (F) and male (M) SHR rats were divided into 8 experimental groups: sham+vehicle (SV), sham+enalapril (SE; 10 mg/kg body weight), castrated+vehicle (CV), and castrated+enalapril (CE). The treatments began 21 days after castration and lasted for 30 days. The ACE and ACE2 activity were measured by fluorimetry. Results: Male rats exhibited a higher values than the female rats in ACE ventricular activity (MSV: 8.47 ± 0.81 AFU, FSV: 5.78 ± 0.79 AFU) and ACE2 (MSV:0.1154 ± 0.017 AFU; FSV: 0.063 ± 0.006 AFU). Regarding ACE activity the castration reduced in males and did not change in female (MCV: 3.72 ± 0.46; FCV: 6.26 ± 0.31). Enalapril treatment both in Sham animals (MSE: 3.99 ± 0.50 and; FSE: 3.91 ± 0.21) as castrated (MCV: 1.94 ± 0.69; FCV: 2.42 ± 0.44) reduced the ACE activity and eliminated the differences between the sexes. In relation to ACE2 activity, in female, none of interventions promoted change in ACE 2 (FSV: 0.063 ± 0.006 AFU; FSE: 0.091 ± 0.010, FCV: 0.095 ± 0.009; FCE: 0.074 ± 0.013). In males the castration promoted reduction in ACE2 (MCV: 0.012 ± 0.003), the enalapril treatment was able to increase ACE2 activity in sham (MSE: 0.167 ± .018) and castrated (MCE: 0.058 ± 0.009) when compared with vehicle values. Conclusion: we observed sexual dimorphism in the plasma and left ventricle ACE activity and in the left ventricle ACE2 activity. Male showed higher activity these enzymes than females. Enalapril treatment reduces the ACE activity regardless of gender and increases ACE2 activity in male, but did not affect in female. The castration, in males, reduced ACE and ACE2, in both plasma and left ventricle depending effects hormones. The effect of enalapril on ACE activity appears to be hormones independent while on ACE2 activity in males appears to dependent on hormones.