Sweating and increased thirst are common symptoms of menopause (Minkin et. al., 1997; Cadena, 2010) and also important factors during exercise (Francesconi, 1988), although thirst sensation decreases with ageing (Ferry, 2006). In this study, we examined the sweat rate (SR), sweat volume (SV), sweat sodium concentration (S[Na+]) and changes in thirst perception (TP), in premenopausal (preM), perimenopausal (periM) and postmenopausal (postM) women after a moderate exercise. Thirty (30) healthy women comprising of preM (aged 22.5±0.8yrs, n=10), periM (aged 46.5±1.1yrs, n=10) and postM (aged 52.2±0.9yrs, n=10) participated in the study. Participants were adequately informed of the experimental procedures and they all consented to it. TP was rated using the Visual Analogue Scale, VAS. Sweat was obtained with the sweat suction apparatus from a 120cm2 circular area marked on the skin of the face and neck (Ugwu and Oyebola, 1996), after a moderate exercise (a 15min walk on the treadmill) at a speed of 4.2 km/h at 27 0C, with the start time noted and SV and [Na+] measured. SR was determined using the formula: (volume of sweat collected)/(exercise time) . Exercise induced a significant change in TP in all groups- preM , periM and postM (2.1±0.5 cm vs 2.2±0.2 cm vs 0.99±0.2 cm), with the postM women exhibiting the least thirst ratings (p<0.05). Although there was no significant difference in SV (1.3±0.4 ml vs 1.7±0.2 ml vs 0.9±0.1 ml) and S[Na+] (125.4±18.7 mmol/l vs 77.2±8.8 mmol/l vs 101.3±10.6 mmol/l) between the groups, the periM women showed a significantly higher SR (0.07±0.02 ml/min vs 0.12±0.01 ml/min vs 0.06±0.01ml/min) (p<0.05). A significant (p<0.0001) and positive correlation existed between TP, SR, SV and S[Na+] . In conclusion, these data are consistent with the earlier reports that older adult women exhibit diminished thirst sensation and further indicate that increase in sweat rate and volume produce a concomitant increase in thirst perception in women during moderate exercise.