Estrogen replacement therapy has been the treatment of choice for menopause symptoms, but it can increase risk of breast cancer, heart disease, and stroke. Due to these side effects, a therapy with phytoestrogens (Wuttke et al, 2002); derived xenoestrogens found in plants is currently being challenged. Moringa (Moringa oleifera Lam.) seed oil (MSO) has been reported to contain phytoestrogens (Anwar et al, 2007). However, it is not known whether and if it can be a treatment of choice for menopause symptoms. Thus, the aim was therefore to study the effects of MSO in menopause using ovariectomized rats (OVX) as a model. The seeds of Moringa were obtained by cold pressed. The animal cares were followed the guidelines of the Committee on Care and Use of Laboratory Animal Resources, National Research Council of Thailand. The procedure of the experiment was performed with the advice of the Institutional Animal Care and Use Committee, Suranaree University of Technology, Nakhon Ratchasima, Thailand. Adult female virgin rats (200-250 g) were overiectomized and divided into 5 groups of six rats each; sham operated rats (SHAM) received 1% tween 80 (1 ml), OVX received estrogen (0.02 mg/kg BW 17β-estradiol), OVX received 1% tween 80 (1 ml; control group), OVX received MSO (0.5 ml/100 g BW), and OVX received MSO (1 ml/100 g BW) orally/day for 6 weeks. At the end of the treatment the animals were sacrificed. The uterus was removed and weighed. Serum estradiol, total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL) and triglyceride concentration were also evaluated. The statistical analysis of differences between groups was performed using one way ANOVA. There was a significant increase in mean relative uterine weight in MSO (1 ml/100 g BW) and estrogen treated-group as compared to the control group (P < 0.05). The results showed that serum estradiol (pg/ml) in SHAM, control group, MSO (0.5 ml/100 g BW), MSO (1 ml/100 g BW) and estrogen treated-group was 39.67±2.2, 13.67±1.5, 20.33±3.1, 50.67±8.9 and 82.00±18.0, respectively. There was a significant increase in serum estradiol with estrogen-treated group and MSO (1 ml/100 g BW) as compared to the control group (P < 0.05). The concentration of total cholesterol, LDL, and triglyceride was not significantly deferent among groups. However, there was a significant increase in the mean HDL in MSO (1 ml/100 g BW) and estrogen treated-group as compared to the control group (P < 0.01). In conclusion, MSO can increase relative uterine weight, serum estradiol, serum HDL in ovariectomized rats. Thus, it may be beneficial for the treatment of menopause symptoms.