This workshop aims to introduce the theory and application of stable isotope tracers and mass spectrometry in physiological research. When combined, these two approaches allow the measurement of the rates at which metabolic processes are occurring, providing insight into the factors and underlying mechanisms that regulate metabolism. This is an opportunity for early career researchers and postgraduate students who may have an interest in understanding or applying these techniques within their work.  

Over the two days, attendees will be introduced to the theory behind these novel isotopic approaches, and to the use of and application of state-of-the-art mass spectrometric analyses. This includes hands-on opportunities for sample preparation and access to a variety of mass spectrometric instrumentation. A basic introduction will be provided to the computational handling and interpretation of the data generated by these techniques.  

At the end of the workshop participants will: 

• Understand the basic theory behind the principles of stable isotope tracers in physiological research.  
• Understand how to design and apply a stable isotope tracer approach to a specific physiological research question.  
• Introduce the analytical approaches aligned to stable isotope tracer techniques in the form of mass spectrometry. 
• Learn how to perform basic analytical and sample preparation for stable isotope tracer studies. 
• Understand how to compute and interpret the raw data and apply the findings to original physiological questions.