The yeast-2-hybrid method was used to examine the interaction between intracellular domains of cation-chloride cotransporters (amino and carboxyl termini) and regulatory proteins. We have identified two Ste20-related kinases as putative interactors with cation-chloride cotransporters (Piechotta et al. 2002). The role of the kinases is analysed in terms of potential phosphorylation of cotransporters, as well as in terms of acting as an intermediate in the activation of MAPK pathways.
University of Manchester (2003) J Physiol 552P, SA1
Research Symposium: Protein-protein interaction and cation-chloride cotransporters
Eric Delpire
Departments of Anesthesiology, Molecular Physiology & Biophysics, Vanderbilt University Medical Center, Nashville, TN, USA
View other abstracts by:
Where applicable, experiments conform with Society ethical requirements.